To investigate the response of corn to combined application of chemical fertilizers with rhizobacteria plant GROWTH promoting, an experiment was conducted in 2017 at Research farm of Agricultural and Natural Resources Campus of Tehran University, Karaj, Iran, in a randomized complete block design with four replications. Four nutritional treatments including T1 (Control treatment without applying fertilizer), T2 (Just PGPRs), T3 (Use chemical fertilizers based on soil test) and T4 (T3 + PGPRs) were considered. According to the results, the highest total dry weight (3.9 kg/m2), crop GROWTH rate (79.8 g.m-2.day-1), net assimilation rate (15.3 g.m-2.day-1) and grain yield (18.2 ton.ha-1) were observed in T4 treatment and T2 treatment produced the highest  leaf area index (5.3), leaf area duration (205.2) and specific leaf weight (78.5 g.m-2) . Also, the lowest value of all traits was observed in in T1 (control) treatment. The results showed that the presence of rhizobacteria plant GROWTH promotioninduction in the corn nutrition program increased the GROWTH and GROWTH indices of the plant. Combined application of chemical fertilizers with rhizobacteria plant GROWTH promoting resulted in the highest GROWTH and final grain yield of corn.

Background: Breast cancer is a multifactorial potentially lethal disease that is triggered by genetic factors as well as numerous environmental factors. The present research aimed to examine the expression of Vascular endothelial GROWTH factor messenger RNA (VEGF mRNA), cytokeratin-19 mRNA (CK19 mRNA), and vascular endothelial GROWTH factor (VEGF) protein biomarker in the peripheral blood of patients with breast cancer.Methods: 40 patients with breast cancer were compared to 40 healthy individuals. The real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) method was used to determine the expressions of the CK19 mRNA and VEGF mRNA biomarkers in the peripheral blood samples of the healthy participants and the patients. The VEGF protein was also compared using the (ELISA) method.Findings: The positive VEGF mRNA biomarker was observed in 30 of the 40 patients with breast cancer; thus the sensitivity of this marker was 75%. In the healthy participants group, 6 of the 40 participants showed a positive VEGF mRNA biomarker expression. The CK19 mRNA marker was positive in 25 of the 40 patients, which indicated a sensitivity of 62.5%. In the healthy participants group, the positive expression of the CK19 mRNA biomarker was observed in 7 of the 40 participants. VEGF was positive in 27 of the 40 patients. In the control group, 5 of the 40 participants showed the positive expression of this biomarker.Conclusion: In sum, based on the results of this research, the assessed breast cancer tumor markers can be used as screening tests for the early diagnosis of patients. To further prove the findings of this study, more extensive studies with larger sample sizes are recommended.

Introduction: The discovery of hidden patterns in pharmaceutical data can contribute to improving the performance of hospital pharmacies. One of the applications of advanced data analysis techniques is the identification of drug interactions. Method: This study was conducted using data mining techniques with the FP-GROWTH algorithm in the RapidMiner Studio® 10.1 environment to extract association rules and frequent pharmaceutical patterns. Data preprocessing and modeling were performed based on the CRISP-DM model. The type and level of drug interactions were determined based on the algorithm's results and by referencing the database at www.drugs.com. Results: The results included 17 association rules and 126 prescribing patterns, ranging from single-drug to four-drug combinations. Of the 64 two-drug prescribing patterns, 56 had no interaction, 6 had moderate interactions, 1 had a minor interaction, and 1 had a major interaction. Additionally, of the 19 three-drug patterns, 18 had no interaction, and only 1 had a moderate interaction. No interactions were observed in the four-drug prescribing pattern. Conclusion: The findings of this study can assist stakeholders in improving the pharmaceutical supply chain, optimizing prescriptions, reducing drug interactions, and lowering costs. The discovered patterns may also be used as part of a clinical decision support system. Although no significant drug interactions were observed in this study, the discovery of even one major interaction highlights its importance and further underscores the practical role of computer applications in medicine.

Extended Abstract Introduction:  Lallemantia royleana is an annual herbaceous plant of Lamiaceae family in different parts of Europe, the Middle East, and especially Iran. Cyamopsis tetragonoloba L. is a plant of the legume family. A common feature between these two plants is hydrocolloid gums, which stabilize some food emulsions by absorbing water and increasing the viscosity or forming a gel in the aqueous phase. Due to its diverse and rich vegetation, Iran can produce countless types of plant gums, and many seeds such as Lallemantia royleana and Cyamopsis tetragonoloba contain valuable gums. Considering the important therapeutic and industrial applications of C. tetragonoloba and L. royleanaplants and the need for more information and reports on determining the best humic acid level and salt stress tolerance of these plants, the purpose of this research is to investigate the tolerance of two L.   royleana and C. tetragonoloba plants to salinity stress in the germination stage and the initial stages of the GROWTH of two plants under the humic acid application. Materials and Methods: The experiment was carried out as factorial in a completely randomized design with three replications at the seed laboratory of the Faculty of Agriculture of Zabol University in 1400. In this experiment, salinity stress was investigated using sodium chloride at control (no salinity), 70, 140, 210 mM levels and humic acid at (0, 40, 80, and 120 mg/L) levels. Humic acid solution at different salinity levels was added to each petri dish containing 25 seeds. Results:  The results showed that salinity stress decreased germination percentage, RADICLE length, plumule length, seedling length and seedling dry weight of C. tetragonoloba. In this plant, the germination percentage decreased by 35. 34% compared to the control as the salinity stress level increased to 210 mM, and with the increase of the stress to more than 140 mM, a significant decrease in the germination percentage was observed. The maximum plumule length of L. royleanaplants was obtained in 70 mM salinity treatment and 40 mg/L fertilizer level. The maximum RADICLE length in the L.   royleanaplants plant was obtained in the treatment of 40 mg/L of humic acid at a 70 mM salinity stress level. Also, the results showed that the maximum RADICLE length (1. 46 cm) in the C.   tetragonoloba plant was related to humic acid pretreatment at 70 and 140 mM salinity and fertilizer levels of 40 and 80 mg/L. Conclusion:  In general, it can be stated that the germination indices significantly decreased under stress conditions, and this indicates that humic acid is a suitable pretreatment that can improve the GROWTH indices of C. tetragonoloba and L.   royleanaplants under stressed and non-stressed conditions. With the application of humic acid at all salinity levels except 210 mM, the germination indices in the two mentioned plants were in a favorable condition. Highlight: 1-The salinity stress tolerance threshold was studied in Cyamopsis tetragonoloba and Lallemantia plants. 2-Humic acid fertilizer in this study mitigated the destructive effects of salinity stress in Cyamopsis tetragonoloba and Lallemantia plants.

Selection based on molecular markers is one of the new methods that may improve progress and accuracy of selection in animal breeding programs. The GHRH gene (GROWTH Hormone-releasing Hormone) is a candidate gene for marker-assisted selection strategies. Polymorphs of GHRH gene are reported to be significantly associated with milk production and constituent traits. In order to study the polymorphism of GHRH gene, blood samples were collected from 112 Sarabi cows. Genomic DNA was extracted and a fragment of 297 bp in size was amplified using polymerase chain reaction. The amplified fragments were subjected to restriction digestion with HaeIII endonuclease enzyme and the resultant digested products were run on 2% Agarose gel. The results revealed the existence of two alleles of GHRHA and GHRHB for the examined locus with frequencies of 0.19 and 0.81 respectively. Three different genotypic variants including GHRHA GHRHA, GHRHA GHRHB and GHRHB GHRHB were identified with genotypic frequencies of 0.0357, 0.3037 and 0.6607 respectively. The  c2 test showed that population is in Hardy-Weinberg equilibrium (P<0.05). These data provide evidence that sarabi cattle breed have a genetic variability, which opens interesting prospects for future selection programs, especially marker-assistant selection.

Unsatisfactory germination of annual canary grass (Phalaris minor) is contributed to seed don11ancy. Factors affecting the breaking of seed don11ancy were investigated to evaluate their effects on the germination of seed samples collected from three different provinces of Iran. Results indicated that the percentage of germination and RADICLE GROWTH rate of seed samples varied significantly. Treating seeds with sulphuric acid, pre-chilling seeds prior to incubation, and placing seeds on filter paper moistened with gibberellic acid at 100 ppm during incubation, all significantly enhanced the germination percentage of the seed samples. Applying a combination of these factors resulted in 90 to 98% seed germination and significant RADICLE elongation. Consecutive freezing and thawing of annual canary grass seed usually had no effect on enhancing seed germination in this weed.